Moreover, cryopreserved semen is easy to store and transfer and makes it possible to perform bioassays in different sites or at different times with the same batch of semen. The test showed good reliability and was found to be rapid and easy to perform, requiring only a small volume of the sample. The sensitivity of the sperm motility parameters used as endpoints (motility percentages and velocities) proved to be comparable to what has been recorded for the fresh semen of other aquatic species (LOECs from 0.02 to 0.03 mg L(-1)). aurata semen to ecotoxicological contamination. In the second phase of the research, bioassays were performed, using cadmium as the reference toxicant, in order to evaluate the sensitivity of cryopreserved S. Motility parameters in the cryopreserved semen did not change significantly in the first hour after thawing, meaning that they were maintained for long enough to enable their use in spermiotoxicity tests. Firstly, the sperm motility pattern of cryopreserved semen was evaluated after thawing by means of both visual and computer-assisted analyses. Cryopreservation is a biotechnology that can provide viable gametes and embryos on demand, rather than only in the spawning season, thus overcoming a limitation that has hindered the use of some species in ecotoxicological bioassays. The aim of this study was to evaluate the feasibility of using cryopreserved S. Additional research should improve the fertility rate and determine how applicable the technique is to other crane species.Ĭryopreserved semen in ecotoxicological bioassays: sensitivity and reliability of cryopreserved Sparus aurata spermatozoa.įabbrocini, Adele D'Adamo, Raffaele Del Prete, Francesco Langellotti, Antonio Luca Rinna, Francesca Silvestri, Fausto Sorrenti, Gerarda Vitiello, Valentina Sansone, Giovanni A 50% fertility rate from most sandhill cranes, Grus canadensis, inseminated with frozen-thawed semen can be expected. Cryopreservation is useful for the long-term storage of crane semen and for specialized propagation needs. The method for the cryopreservation of crane semen at Patuxent Wildlife Research Center is described in detail. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P semen freezing reduced the rate of in vitro-produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 Ã- 10 6 spermatozoa/mL and frozen with SP (2) centrifugation (C) at Ã-600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. The removal of SP may improve the quality of frozen bull semen. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility.Ĭampanholi, Suzane Peres Monteiro, Fabio Morato Ribeiro Dias, Erika Aline Mercadante, Maria Eugênia Zerlotti de Paz, Claudia Cristina Paro Dell'Aqua Junior, José Antonio Papa, Frederico Ozanam Dell'Aqua, Camila de Paula Freitas Vantini, Roberta Garcia, Joaquim MansanoĬryopreservation of bull semen is a common biotechnology procedure in cattle breeding.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |